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Qualitative pollen analysis of honey from bees kept in Czech Republic
VALEŠKOVÁ, Denisa
Pollen analysis is the determination of a pollen grains in a honey sediment. The main goal of the thesis was to qualitatively assess a honey from the bees breed in different parts of the Czech republic and to evaluate, if there are any differences in bee grazing between a bees breed in the city and a bees breed in the countryside. Investigate, if a pollen is associated with the colony diseases and higher susceptibility to the pesticides from the field crop. It was found that the dominant plant taxon were canola and fruit trees. In the urban centers was high concentration of the pollen grains from a woody plants, which bees probably visit in local parks. Rural areas were miscellaneous species with high concentration of the herbaceous plants that are part of the meadows.
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The mechanisms of pollen incompatibility in the Brassicaceae family
Šesták, Petr ; Fíla, Jan (advisor) ; Vosolsobě, Stanislav (referee)
Sporophytic incompatibility (SI) represents one of the systems by which angiosperms prevent pollination by their own pollen or by the pollen from a genetically related plant. It is mostly studied in the Brassicaceae family, mainly due to its agricultural importance. Another reason is that the model plant Arabidopsis thaliana belongs to this family. In the last three decades, advances in molecular biological methods enabled the characterization of a large part of the signalling cascade that leads to the rejection of incompatible pollen. Then, the functions of various cellular components (e.g. cytoskeleton, exocyst or proteasome) involved in the incompatible response to pollination are studied mainly by live cell microscopy. Last but not least, the function of SI under various abiotic stresses was described to reveal their influence on SI mechanisms. The aim of this bachelor thesis is to summarize the new discoveries characterizing the molecular mechanisms of SI in the Brassicaceae family, to describe the processes leading to the germination of compatible pollen grain and to characterize the newly described proteins involved in cellular signalling leading to the rejection of incompatible pollen.
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Important plants in terms of pollen for bee-keeping in the spring and early summer in the "Zbudovska Blata" region
ŠOLÁ, Jitka
Objective of the theses was to determine the botanical origin of bee-collected pollens using pollen analysis. The beehive, from where the pollen came from, is located in Hlavatce, which is 20 km far from České Budějovice. Bee-collected polens was obtained via pollen trap, which was put on the beehive once a week for 24 hours between 17th March to 6th June 2017. Pollens were dried, sorted according to the color shades, and the partial color samples were weighed on the analytical scales. The pollen analysis took place at a microscope magnification of 400x. The characteristics of exine (sculpture) and quantity and type of apertures were observed. The size of the grain, which was measured always in 50 pollen grains, helped to classify the plant species. Using the Shannon-Wiener Diversity Index, the bee's preferences were assessed in individual samples. The work is supplemented with photos of selected pollen grains.
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Pollen charakterisation in size segregated aerosol
Kolpakova, Anna ; Hovorka, Jan (advisor) ; Zíková, Naděžda (referee)
This bachelor thesis, in literature review, deals with bioaerosol issue, describes meaning of pollen, ways of pollen spreading and its influence on human health, summarize methods of pollen sampling from the air and common ways of pollen grains identification. In the experimental part, the use of the first stage of a High Volumetric Cascade Impactor (Hi-Vol) BGI-900 for bioaerosol sampling and subsequent SEM analysis was proved. Method of pollen separation from the impaction substrate, polyurethane foam, into homogeneous deposit on Nylon filter was optimized. Representative portion of the deposit was analyzed by SEM. There were taken 485 SEM pictures from 12 samples in 3 localities in the ČR. Pollen grains were identified in 295 SEM and determined into 9 genus and 4 families and grain deformities were quantified to be 24, 18, 50% for Prague, Brezno and Láz localities. Number of pollen increased with total aerosol mass in Prague locality only. Thre aleo identified insect secrction products borochomomes in the samples. Key words: pollen, pollen grain, bioaerosol, cascade impactor, SEM.
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The importance of production and quality of pollen in the summer and late summer for the bee colonies (case studies from around of the village Volary - CHKO Šumava)
PETROVÁ, Jana
Objective of the work was to identify with using pollen analysis botanical origin of bee-colected pollens collected from beehive station Lískovec near the town Volary in the Protected Landscape Area of Šumava. Bee-collected pollens were collected once a week from one honey bee colony with using of a pollen trap. The time of collection was summer and late summer period from 22 June to 11 September 2010. Bee-collected pollens were dried, categorized by color, weighed and observed using the microscope. Pollen grains were determined by the number and type of apertures, shape, sculpture and size. Size of pollen grains was measured on at least 50 pollen grains of one type. In each microscopic slide were counted at least 500 pollen grains. The proportion of plant species in the sample was expressed as a percentage and the importace of plant species was evaluated in the diet of honey bees in the area of interest. Selected samples of bee-collected pollens from beehive station Lískovec and also from beehive station Dobčice were investigated for protein content and amino acid composition. Pollen belonging to Polemonium coeruleum, Trifolium pratense and Trifolium repens was evaluated as an important source of protein and amino acids for honey bees. The concentration of essential amino acids expressed as percentages of total amount of amino acids did not differ significantly between samples of bee-collected pollen. Another objective of the work was to compare the results from the area of interest with the results processed in the similar study from beehive station Dobčice in the northern area of the landscape Blanský les. The work also includes statistical evaluation of dominance, frequency, Shannon-Wiener index of diversity and variability between samples and species. The work is complemented by photographs of selected pollen grains.
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Important plants in terms of pollen for bee-keeping in the spring and early summer in the area of the protected landscape Šumava
ŠEMRO, Martin
Objective of the work was to identify with using pollen analysis botanical origin of bee pollens. Pollen analysis consisted in the distribution of samples to individual sub-samples . Samples were weighed and a part of them was dissolved in a solution of glycerin and water. Dissolved samples were viewed under a microscope. Pollen was removed by the beekeeper Mgr .Milan Trhlín with using a device called pollen catcher. The samples were collected in the year 2011 in the spring and early summer period from beehive location on the hill Lískovec, of the protected landscape Šumava 1,8 km west of city Volary, at a time of 25. march to 24. june. Another objective of the work was with using phytocenology study, in the distance 1,5 km from the beehive habitat, evaluate the structure of plant association from the point of nutrition honeybees. The work also includes evaluation preference bees for individual plant species. There were detected, that bees prefer the plants that provide the best digest pollen and the plants which grows up to 1,5 km from beehive habitat. There were distinguised 32 types of pollen grains.
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Important plants in terms of pollen for bee-keeping in the summer and late summer aspect in the northern area of the landscape Blanský les
PETROVÁ, Jana
Objective of the work was to identify botanical origin of bee pollens collected from the northern territory of Protected Landscape Area Blanský les by pollen analysis and assess the importance of individual plant species in the diet of honey bee in the range up to 2 km from the beehive. The work also includes statistical analysis, which examined how rich is the preference of bees in the collection of bee pollens, as well as the occuring of plant taxa in the proportion of samples and what is the similarity of sampling and individual plant taxa in terms of preference of bees. There were counted 23 766 different pollen types and plant taxa of bee pollens collected during summer and late summer between 2010 and 2011. The work is complemented by photographs of selected pollen grains.
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